Camptothecin hl-60

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WebOct 1, 2001 · The study identifies and delineates signaling factors involved in BN80915-induced apoptosis in HL-60 cells. Cell cycle analysis of HL-60 cells treated with graded … WebJun 1, 1992 · Camptothecin or teniposide induced apoptosis selectively in S phase of HL-60 cells; apoptotic cells from camptothecin-treated cultures, however, despite the marked nucleolysis, still expressed p120.

Cleavage of Bax to p18 Bax accelerates stress-induced apoptosis, …

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WebFeb 1, 2008 · Camptothecin HL-60 cells Apoptosis Cell cycle 1. Introduction Phospholipase D (PLD) is an enzyme that hydrolyzes phospholipids. In mammalian cells, there are two PLD isoforms, PLD1 and PLD2 that share significant sequence similarity, but have distinct subcellular locations and activity. WebJun 1, 2000 · Camptothecin (50 mM), a cytotoxic quinoline alkaloid which inhibits the DNA enzyme topoisomerase I, was used as a positive control (King et al., 2000). ... Internalization of Bacillus intermedius... WebAug 1, 1999 · HL-60 human promyelocytic leukemia cells were cultured in RPMI 1640 supplemented with 10% fetal bovine serum. For induction of apoptosis, cells were exposed for 3 h to 0.1 μg/ml DNA topoisomerase I inhibitor, camptothecin. Quantitative analysis of apoptosis was carried out by flow cytometry. high school furniture

New camptothecin derivatives for generalized ... - ScienceDirect

WebThere have been some conflicting results concerning whether annexin V binds to camptothecin (CAM)-treated HL-60 cells, a commonly used model for apoptosis. We investigated the effects of culturing HL-60 cells for up to 8 h with a range of CAM concentrations. METHODS We used flow cytometry to measure cellular light scatter, … WebCamptothecin is a plant alkaloid present in wood, bark, and fruit of the Asian tree Camptotheca acuminata. From a shaky beginning complicated by toxic effects and logistical issues, camptothecin derivatives have advanced to become standard components in the treatment of several malignancies. how many cherry tomatoes in 1 pintWebDNA single- strand breaks were detected using alkaline sucrose gradient centrifugation when HL-60 cells were incubated with 10 μMCPT or 10 μMSN-38 for 30 min. These … high school gala

"WebJun 13, 2024 · Camptothecin is an anticancer molecule which acts by targeting the human deoxyribonucleic acid topoisomerase I (Topo-I). It inhibits the enzyme by blocking the re-joining step of the cleavage reaction of Topo-I, which causes accumulation of a covalent reaction intermediate. " - Camptothecin hl-60

Camptothecin hl-60

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WebJun 29, 2015 · ；D. 大黄素无法捕获Topo I-DNA 的可切割复合物，Topo 的抑制剂CPT作为阳性对照；大黄素能够诱导稳定的TopoII α-DNA 可切割复合物，Topo II 的抑制剂VP16 作为阳性对照。G. 用大黄素处理HL-60 和HL-60/MX2 细胞后，检测γ -H2AX 的表达。H. 大黄素和VP16在Topo II –缺陷型细胞HL ... WebAug 15, 2024 · The IC 50 values on the HL60 cells are comparable to SN38 and 9-N - (2′-hydroxyethyl)methyl substituted compound 2b. Table 1. IC 50 values of Irinotecan (1c), SN38 (1d) and SN38 derivatives 2a-j. nontoxic - lack of cell viability decrease/toxicity in the studied compound concentration range.

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WebCamptothecin is a naturally occurring alkaloid derived from the plant Camptotheca acuminata that was identified in an anticancer drug discovery screen in the 1960s.67 … WebThe meaning of CAMPTOTHECIN is an alkaloid C20H16N2O4 from the wood of a Chinese tree (Camptotheca acuminata of the family Nyssaceae) that has shown some …

WebCamptothecin, a cytotoxic plant alkaloid with antitumor properties, is a prototypic DNA topoisomerase I inhibitor. ... This camptothecin concentration range works well for inducing Jurkat or HL 60 cell suspensions which usually run in the 1 x 105 – 1 x 106 cell/mL concentration range. At this concentration range successful apoptosis induction ... WebNuclear PI-PLC activity in HL-60 cell nuclei after treatment with camptothecin Source publication The pro-apoptotic drug camptothecin stimulates phospholipase D activity and diacylglycerol...

WebThe Aerospace Maintenance Competition is an annual celebration of aviation maintenance technicians and their skills. Held during Aviation Week at the MRO Ame... WebCamptothecin is a naturally occurring alkaloid derived from the plant Camptotheca acuminata that was identified in an anticancer drug discovery screen in the 1960s. 68 …

WebOct 1, 2003 · (A) HL-60 cells were treated with 2 μM camptothecin only or in the presence of 20 μM calpeptin (calpain inhibitor), or 20 μM cathepsin inhibitor I (CI) or 20 μM ALLM (inhibits calpain and cathepsin) for the indicated times. (B) HL-60 cells were treated with 20 μM cisplatin, or 50 μM etoposide or 0.5 μg/mL adriamycin alone for 20 hours or ...

WebCamptothecin [47, 48]. binds non-covalently with topoisomerase-I and suppresses their catalytic activity, thus show- Vinblastine and its derivatives have been used ing anti-cancer properties. ... (HL-60, MCF-7) with IC50 value Due to the large diversity of endophytic fungi, (5.8, 20.15 μM) [92]. Incarxanthone B, a novel various novel cytotoxic ... how many cherubim angels are thereWebNov 5, 2015 · The effect of vincristine, cisplatin or 10-hydroxy camptothecin on inducing apoptosis of HL-60 cells co-cultured with the MS-5 cell layer within 48 h was tested with/without E5. high school game 4-2WebApr 27, 2010 · Polysaccharopeptide (PSP) from Coriolus versicolor (Yunzhi) is used as a supplementary cancer treatment in Asia. The present study aims to investigate whether PSP pre-treatment can increase the response of the human leukemia HL-60 cells to apoptosis induction by Camptothecin (CPT). We used bivariate bromodeoxyuridine/propidium … high school gainesville flWebWe investigated expression of cyclin A in HL-60 cells after induction of apoptosis with doxorubicin and etoposide. Following apoptotic trigger, both cells arrested in G2/M phase of the cell cycle... high school game footballWebSep 1, 2001 · To shed light on the mechanism of action of hCPT at the cellular level, we compared the effects of BN80915 and the classic camptothecin SN-38, the active metabolite of irinotecan, on HL-60 human promyelocytic cancer cells. how many chesapeake shores seasons are thereWebAug 1, 1993 · Since apoptosis has been associated with potential involvement of intracellular signaling linked to the Ca 2+ /calmodulin and protein kinase C transduction … high school game freeWebCamptothecin is an alkaloid first isolated from the stem wood of the tree Camptotheca acuminata (Nyssaceae), and has potent antitumor activity. 31 Camptothecin acts by … high school galveston