WebTo quantify the total phenolic content (TPC) present in each extract, the Folin–Ciocalteu (FC) method was used [37,53]. This method is mainly based on the redox reaction at basic pH of the total phenolic compounds present in the extract with the Folin–Ciocalteu reagent, which, after being reduced by the phenolic groups present, turns from a ... WebThe antioxidant capacity was associated with the phenolic content of the wines, determined either by Folin-Ciocalteau or by summation of the individual phenolics determined by HPLC or by spectroscopy. Total phenolics as measured by the Folin-Ciocalteau method ranged from 6.47 to 18.6 mM GAE per liter. The anthocyanin levels ranged from 101.5 to ...
Determination of total phenolic content and antioxidant ... - PubMed
WebNov 29, 2012 · Several methods have been described for the determination of phenolic compounds in animal and plant products using the Folin-Ciocalteu (FC) assay. Most of these methods describe the use of this reagent and sodium carbonate in spectrophotometric methods. The macro FC assay was compared with two micro FC assays carried out on a … WebJun 30, 2012 · The FC test was carried out according to the following procedure: 1 mL of sample (wines were diluted 1:100 or 1:50), 6 mL of deionized water, 0.5 mL of Folin–Ciocalteu reagent and 2 mL of a 20% sodium carbonate solution were added in this order to a 10 mL beaker and diluted to volume with deionized water. hwq16 pool heater control
Effect of esterification condensation on the Folin–Ciocalteu method for
WebApr 12, 2024 · Total polyphenol content was determined by the Folin–Ciocalteu method [23,24,25] partially modified for application to small samples. A 1.5 mL microtube was charged with the crude extract (2 μL) and Milli Q water ... (Multiskan FC, Thermo Fisher Scientific, Waltham, MA, USA), and the total polyphenol content was calculated from this ... WebJan 28, 2005 · An optimized Folin-Ciocalteu method to specifically determine the contents of total polyphenols and vitamin C is reported, set up with synthetic solutions and validated on different extracts from fruit products. Polyphenols, widely spread in our diet by the consumption of plant food products, are commonly determined using Folin-Ciocalteu … WebAug 2, 2024 · The extraction procedure described for Oasis ® MAX cartridges was applied, and 15 μL of the eluted fractions were mixed with 170 μL of Milli-Q water in the thermos microtiter 96-well plate, adding 12 μL of Folin-Ciocalteu (FC) reagent, and 30 μL of sodium carbonate (200 gL −1). The mixtures were incubated for 1 h at room temperature in ... masham tennis club