Gfp hepg2 cells
WebBottom: HepG2 cells were transfected with a plasmid expressing GFP, and visualized using either visible light or fluorescence. Experiment 3: Comparison of EndoFectin™ HepG2 and electroporation EndoFectin™ HepG2 can also provide nearly as high transfection efficiency as electroporation, meaning that you can achieve ultra-high levels of ... WebHepG2-preS1-GFP cells were cultured in R10 culture medium as follows. 1. Prepare R10 medium by adding to RPMI 1640 10% of heat inactivated fetal bovine serum, 20 mM HEPES, 1 mM sodium pyruvate, 100 IU/mL penicillin, 100 μg/mL streptomycin, MeM amino acids, Glutamax, MeM non-essential amino acids, 5 μg/mL Plasmocin. 2.
Gfp hepg2 cells
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WebEach reagent was used to transfect HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines in a 96-well format, and GFP expression was analyzed 48 hours post-transfection. … Web2 days ago · HepG2 cells were cultured in 75 cm 2 culture flasks at a density of 5 × 10 6 cells per flask and grown to 80% confluence. The cells were treated with CA4G (10, 1, and 0.1 μmol/L), FA4G (10, 1, and 0.1 μmol/L) or SC79 (10 μmol/L) for 6 h. ... We performed membrane translocation assays of the AKT PH domain with the help of the PH-GFP …
WebHepG2 cells are adherent, epithelial-like cells growing as monolayers and also in small aggregates. About turboGFP protein: tGFP is an improved variant of the green fluorescent protein CopGFP from copepoda … WebGeneral Transfection Protocols Cell-Specific Transfection Protocols Transfection Reagents - Product Protocols NOTE: Invitrogen Lipofectamine Transfection Reagent Protocols have been optimized for efficiency, viability, and reproducibility across a broad range of cell types. This is often the best place to start, especially in a new cell line.
WebCatalogue NO: cAP-0053GFP, Product Name: GFP Expressing Human HepG2 Cells, High Quality-Price Ratio Products and Services. Quality Assurance Guaranteed. Fast … WebMay 26, 2015 · The expression of yieF was measured in stably transfected cells HepG2-YieF by quantitative RT-PCR and found up-regulated by 3.89-fold upon Cr(VI) induction. …
WebDec 1, 2024 · Human HepG2 cells, established from a differentiated hepatoma, provide a widely used model system for the study of cell polarization ( 2 ). To understand the mechanism for hepatocyte polarity establishment, we used HepG2 cells in 3D culture with ECM-rich Matrigel.
WebHCT 116. CCL-247 ™. HCT 116 cell line was isolated from the colon of an adult male with colon cancer. It has a mutation in codon 13 of the ras proto-oncogene and can be used as a positive control for PCR assays of mutation in this codon. Product category. roman amphitheatres in the ukWebTransfection efficiency of TransfeX Reagent on HepG2. HepG2 cells were prepared and seeded at 2.5 x 105 cells/well in a 12 well plate at 98% viability. HepG2 cells were transfected with EF1α-eGFP vector at 1.0 µg DNA with 2.0 µL of reagent (1:2) in Opti-MEM I Reduced Serum Media. No significant signs of cellular toxicity were observed. Phase ... roman amphoraWebMar 25, 2024 · g, Live-cell imaging of HEPG2 cells that were incubated at 37 °C for 1 h with 50 nM rabbit IgG-647 and 25 nM goat anti-rabbit, goat anti-rabbit M6Pn or goat anti-rabbit GalNAc. Scale bar, 20 µm ... roman amphitheatres in spainWebExperimental result: HepG2 cells were successfully transfected with pcDNA3.1-GFP plasmid using X-tremeGENE™ 9 DNA Transfection Reagent. Transfection of Neuro-2a Cells. Protocol provided by a customer. Plate Neuro-2a cells at a … roman analysisWebJul 21, 2024 · HepG2 cells (from a human hepatoma) were cultured at 37 °C, ... 50 and 100 MOI of the GFP adenovirus and 24 h post-infection the number of GFP positive cells was evaluated. Primary hepatocytes ... roman and anna youtube videosWebOct 14, 2014 · Cells successfully transfected with GFP were separated by flow cytometry (FACScan; Becton Dickinson, San Jose, CA), and the purified, GFP-positive cell lines were named HepG2-199a and HepG2-NC (NC, negative control). ... MTT assays showed that HepG2 cells with increased miR-199a expression (HepG2-199a) proliferated at a slower … roman and alejandra show in hemetWebI have made a couple of HepG2 GFP-reporter cell lines using a custom-made plasmid. After monoclonal selection and amplification, sequencing confirmed the insertion of the correct … roman anatomy