Phospho-histone h2a.x ser139 cell signaling
WebDescription: The PathScan® Phospho-Histone H2A.X (Ser139) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous … WebUse Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, FITC conjugate (mouse monoclonal antibody) validated in FC, ICC to detect phospho-Histone H2A.X (Ser139) also known as H2AXS139P, H2AX h More>> MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. SDS CoA …
Phospho-histone h2a.x ser139 cell signaling
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WebPhospho-H2A.X (Ser139) Antibody detects endogenous levels of H2A.X only when phosphorylated at Ser139. Species Reactivity: Human, Mouse, … WebPhospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb an Antibody by Cell Signaling Technology. Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 an Antibody by Invitrogen Antibodies Goat anti ...
WebH2A.X is phosphorylated at Ser139 by DNA-PK in response to cell death receptor activation, c-Jun N-terminal Kinase (JNK1) in response to UV-A irradiation, and p38 MAPK in response to serum starvation (5-8). H2A.X is constitutively phosphorylated on Tyr142 in undamaged cells by WSTF (Williams-Beuren syndrome transcription factor) (9,10). WebMonoclonal Antibody for studying H2AX (Ser139) phosphate. Cited in 1758 publications. Validated for WB, IHC, IHC, IF, F. Available in 2 sizes. Highly specific and rigorously …
WebHistone H2AX phospho Ser139 antibody tested by Western blot. Western blot: Nuclear extract of U2OS cells (20 µg per lane) probed with Histone H2AX phospho Ser139 polyclonal antibody (1:500 dilution). Lane 1: untreated cells Lane … WebPhospho-gamma H2A.X (S139) ELISA Kit (ab279816) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated gamma H2A.X protein in your experimental model system, you can verify pathway activation in your cell lysates.
WebCell culture. Human keratinocytes HaCaT cells (T0020001; ... (1:1000, 3192), Phospho-eIF2α (Ser51) (1:1000, 9721), ATF4 (1:1000, 11815), BiP (1:1000, 3183) and Phospho-Histone H2A.X (Ser139) (1:1000, 9718), from Cell Signaling Technology®; ATF6 (1:1000, sc-22799), eIF2α (1:1000, sc-133132), and β-actin (1:1500, sc-47778) from Santa Cruz ...
Webof Histone H2A.X protein phosphorylated at Ser139. A Histone H2A.X rabbit antibody has been coated onto the microwells. After incubation with cell lysates, both phospho- and non-phospho-Histone H2A.X proteins are captured by the coated antibody. Following extensive washing, a phospho-Histone H2A.X (Ser139) mouse antibody is added to detect the ... phillip beckwith ddsWebPhospho-histone variant H2A.X (pH2A.X) increased 2-fold in healthy donor PBMCs at 1 nmol/L, whereas the same effect in the melanoma cell line M229 required 10 nmol/L. pH2A.X was inhibited slightly in the PBMCs of 3 patients with metastatic melanoma at 1 nmol/L and in the melanoma cell line M370 at 10 nmol/L. Panobinostat inhibited phospho … trymer mars coat king home 915e - 1 5 mmWebNov 15, 2024 · Cells were permeabilized with ice-cold 0.5% Triton X-100 for 5 min and then washed with PBS. Cells were then incubated with phospho-histone H2A.X (Ser139) antibody (from Cell Signaling Technologies 9542, Boston, MA, USA) for 1 h, followed by DAPI (Sigma-Aldrich, Darmstadt, Germany) for 3 min and washed with PBS/0.05% Tween. phillip beggsWebH2A.X is constitutively phosphorylated on Tyr142 in undamaged cells by WSTF (Williams-Beuren syndrome transcription factor) (9,10). Upon DNA damage, and concurrent with … try mermaidWebThis H2A.X antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml. The antibody was purified by affinity chromatography. Upon receipt, store between 2°C and 8°C. Each lot of this antibody is quality control tested by Western blotting. phillip beck mdWebHistone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle. In eukaryotes, DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX. The phosphorylation of H2AX can … phillip beggs attorneyWebPhospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb detects endogenous levels of H2A.X only when phosphorylated at Ser139. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser139 of human … phillip beeson state farm