Smurf1 antibody

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August undefined, 2024

Web12 Apr 2024 · After sealing with 5% nonfat powdered milk for 2 h, primary incubation was performed overnight at 4°C with primary antibodies at a dilution of 1:1000. And then membranes were probed with an anti-rabbit or anti-mouse HRP-conjugated second antibody at 37°C for 2 h following washing three times with TBST. ... Smurf1 is a target of miR-340 … Web14 Mar 2024 · Smurf1 interacts with Wee1. (A) Co-immunoprecipitation (IP) of endogenous Smurf1 and Wee1. HeLa cell lysates were immunoprecipitated with Smurf1 antibody or IgG antibody and were analyzed by immunoblotting as shown (B) Co-IP was performed in HEK293T cells transfected with Smurf1, RhoA and Wee1 overexpression plasmids.

Smurf1 Antibody Cell Signaling Technology

WebSmurf1, a member of the HECT family of E3 ubiquitin ligases, selectively interacts with BMP pathway Smad effectors, leading to Smad protein … Web26 Dec 2006 · To characterize the role of Smurf1 in MDAMB-231 breast cancer cells, we reduced its expression by using siRNAs or short hairpin RNAs (shRNAs) targeting different sequences in the smurf1 gene (). siRNA or shRNA silencing was accompanied by cell rounding, a reduction in size, and a loss of membrane protrusions.Accordingly, silenced … bakugou hair

Smurf1 regulates tumor cell plasticity and motility through …

Web1 Oct 2012 · Axon growth is an essential event during brain development and is extremely limited due to extrinsic and intrinsic inhibition in the adult brain. The E3 ubiquitin ligase Cdh1-anaphase promoting complex (APC) has emerged as an important intrinsic suppressor of axon growth. In this study, we identify in rodents the E3 ligase Smurf1 as a novel … WebSMURF1 antibody (ab57573) used in immunohistochemistry at 2ug/ml on formalin fixed and paraffin embedded human stomach. This image was generated using the ascites version … WebSMURF1 Antibody (H00057154-M01) in ICC/IF Immunofluorescence of monoclonal antibody to SMURF1 on HeLa cell. Antibody concentration is 10 µg/mL. Product Details … bakugou hair transparent

Monoclonal Anti-SMURF1 antibody produced in mouse

Anti-SMURF1 antibody [1D7] (ab57573) Abcam

WebSmurf1 promotes the ubiquitination and degradation of Nrf2. (a) Smurf1 directly interacts with Nrf2 was assessed by CO-IP. (b, c) Western blot analysis of Nrf2 protein stability in Smurf1-OE LF cells treated with 25 ug/ml CHX for various times. . (d) Cell lysates from control and Smurf1-OE LF cells was immunoprecipitated with anti-Nrf2 antibody, then … Web25 Mar 2024 · Western blot analysis of SMURF1 expression in rat brain extract (lane 1) and MCF-7 whole cell lysates (lane 2). SMURF1 at 86KD was detected using rabbit anti- SMURF1 Antigen Affinity purified polyclonal antibody (Catalog # PB9892) at0.5 ??g/mL. bakugou hugging dekuWebFind high quality SMURF1 tools for research. Antibodies, ELISA kits, proteins, reagents. Order quickly and easily at antibodies-online.com arenera tabar

"Web2 Nov 2024 · Predicted targets including Furin, a protease that cleaves pro-forms, genes encoding receptors such as Acvr2a, Bmp1a, Dies1, and Tgfbr3, molecules within the cascade like Smad5, transcriptional regulators like Ski and Zfp423 as well as Mapk3 and Smurf1 were validated by quantitative PCR. " - Smurf1 antibody

Smurf1 antibody

Web8 Jun 2024 · Abstract. We aimed to investigate the role of cMet agonistic antibody (cMet Ab) in preventing kidney fibrosis during acute kidney injury (AKI) to chronic kidney disease (CKD) transition. Additionally, we explored the effect of cMet Ab on TGF-β1/Smad pathway during the pathogenesis of kidney fibrosis. A unilateral ischemia–reperfusion injury (UIRI) … WebBuy smurf antibodies from Santa Cruz Biotechnology, Inc. Monoclonal antibodies are available to most protein immunogens. Choose a Store Santa cruz biotechnology. Santa …

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Web24 Feb 2024 · DDIT3 promoted NF-κB-dependent ovarian tumor (OTU) deubiquitinase 1 (OTUD1) expression. Furthermore, OTUD1 induced upregulation of the E3 ubiquitin ligase Smurf1 by deubiquitinating Smurf1, and Smurf1 degraded MAVS in MDBK cells in a ubiquitination-dependent manner, ultimately inhibiting IFN-I production. WebSMURF1 is downregulated in Th0 cells in peripheral blood of patients with SLE. • SMURF1 represses the polarization of Th0 cells toward Th17 and Th17.1 phenotypes. • SMURF1 promotes the ubiquitination and decreases the stability of RORγt. Keywords Systemic lupus erythematosus SMAD specific E3 ubiquitin protein ligase 1 Th17 Th17.1

Web21 Mar 2024 · SMURF1 (SMAD Specific E3 Ubiquitin Protein Ligase 1) is a Protein Coding gene. Diseases associated with SMURF1 include Geotrichosis and Brachydactyly, Type A2 . Among its related pathways … WebSMURF1 antibody (ABIN1936804). Validated for IHC, ELISA, WB. Tested in Human, Mouse. Order online. English ... Comparison List Basket Phone: +1 877 302 8632 Fax: +1 888 205 9894 (Toll-free) E-Mail: [email protected]. Home Antibodies ELISA Kits Primary Antibodies. Monoclonal Antibodies; Polyclonal Antibodies; Recombinant Antibodies ...

WebWe have a full line of high quality products such as antibodies, proteins, cell lines, cell culture tools, and modulators; our customized services cover every aspect in the neuroscience field for your tailored needs. ... Attenuates Smurf1-mediated Smad 1/5 degradation and enhances BMP signaling. Potentiates BMP-2-induced osteoblastic activity ... WebDetection of Human SMURF1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human SMURF1 Monoclonal Antibody (Catalog # MAB9507) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018).A specific band was …

Web19 Jan 2024 · Smurf1 Antibody (45-K) is a mouse monoclonal IgG 2a κ Smurf1 antibody, cited in 32 publications, provided at 100 µg/ml. raised against an internal portion of …

Web29 Dec 2024 · Smurf1 and RhoA could be detected in the whole cell lysates of all groups, suggesting that the whole cell lysates could be used for subsequent Co‐IP experiments. When Smurf1 antibody was used to pull down the protein complex, RhoA expression was detected in the control group (Smurf1−/RhoA−), while Ub expression was low. bakugou hintergrund pcWeb17 Jun 2011 · Supplemental Figure 2. Refined structures of Smurf1 WW1-WW2 complexes (A). 15-Refined NMR structures of the Smurf1 WW1-WW2 pair (residues 232-314) bound to the 208-233 segment of the Smad1 linker di-phosphorylated at S210 and S214. The structure was fitted to either the WW1 domain (left image; 0.7 Å for backbone heavy atoms of the … bakugou hintergrundWebSMURF1 Antibody - BSA Free Summary Applications/Dilutions Packaging, Storage & Formulations Alternate Names for SMURF1 Antibody - BSA Free E3 ubiquitin ligase SMURF1 EC 6.3.2 EC 6.3.2.- hSMURF1 KIAA1625 KIAA1625E3 ubiquitin-protein ligase SMURF1 SMAD specific E3 ubiquitin protein ligase 1 SMAD ubiquitination regulatory factor 1 bakugou husbandWebScientific researcher with extensive experience in protein and antibody engineering. My greatest asset is being an effective leader which allows me to work with groups from multi-disciplinary backgrounds. ... Engineering protein-based inhibitors (ubiquitin variants) targeting SMURF1 and SMURF2, members of the ubiquitin proteasome system, which ... bakugou holding penWeb17 Apr 2024 · Smurf1 deficient ameliorates HFD-induced lipid accumulation. A, ... transfected with Flag-Fbw7a and Myc-SREBP-1c deletion mutants were immunoprecipitated and immunoblotted with indicated antibodies. F, Endogenous Smurf1 proteins were immunoprecipitated from the indicated tissue lysates and then detected by Western blot. … bakugou hd wallpaperWebAll Deafness, congenital, unilateral or asymmetric; DEEAH syndrome . All DEEAH syndrome; Primary Antibodies bakugou imagemWeb26 Nov 2012 · Precipitates were then resolved by 4–12% SDS-PAGE and immunoblotted with anti-ubiquitin and anti-SMURF1 antibodies, respectively. SMURF1 and Tubulin levels were also shown by immunoblotting in 1% Input. C, 0.5 μg of pcDNA4/TO-SMURF1 cDNA was co-transfected with increasing amount of V5-tagged USP9X construct (0, 1, 2, and 5 μg) into … arenes san juan lunel